For the trace determination of elements in spinach a new differential pulse polarographic (DPP) method is established. The polarograms were taken in various electrolytes and pH values in the presence of digested spinach sample. Trace element determinations were made in acetate and ammonia electrolytes at various pH values, with or without EDTA. Fe and Cu peaks could be separated and determined at pH 7, acetic add electrolyte in the presence of EDTA. Ni and Zn peaks could be separated and determined in ammonia buffer at pH 10. Lead could be determined at pH 2 acetic acid and Mo (VI) was determined at pH 4 acetate buffer, EDTA medium. Selenite was determined from its hydrogen catalytic peak. The trace element in spinach was found as Fe 15 mu g/g, Cu 17 mu g/g, Ni 8 mu g/g, Zn 27 mu g/g, Pb 47 mu g/g, Mo 43 mu g/g, and Se 762 mu g/g. (C) 2016 Elsevier B.V. All rights reserved.