Seminal plasma composition and its relationship with physical spermatological parameters of Grass carp (Ctenopharyngodon idella) semen: with emphasis on sperm motility


Bozkurt Y., Oegretmen F., Ercin U., Yildiz U.

AQUACULTURE RESEARCH, vol.39, no.15, pp.1666-1672, 2008 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 39 Issue: 15
  • Publication Date: 2008
  • Doi Number: 10.1111/j.1365-2109.2008.02041.x
  • Journal Name: AQUACULTURE RESEARCH
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1666-1672
  • Keywords: sperm motility, seminal plasma, sperm quality, Ctenopharyngodon idella, RAINBOW-TROUT, ONCORHYNCHUS-MYKISS, ATLANTIC SALMON, CYPRINID FISHES, SPERMATOZOA, MILT, METABOLISM, QUALITY, PH, SPERMATOGENESIS
  • Gazi University Affiliated: Yes

Abstract

In this research, the mineral and organic composition of the seminal plasma, physical spermatological parameters and their physiological relationships were investigated in grass carp (Ctenopharyngodon idella). The seminal plasma contained 98.14 +/- 5.23 mM L(-1) (Na(+)), 380.85 +/- 25.95 mM L(-1) (K(+)), 30.25 +/- 4.96 mg dL(-1) (Ca(2+)), 19.16 +/- 1.70 mEq L(-1) (Mg(2+)), 1.36 +/- 0.11 mg dL(-1) glucose, 0.37 +/- 0.08 g dL(-1) total protein, 12.02 +/- 1.18 mg dL(-1) cholesterol, 14.85 +/- 1.50 mg dL(-1) triglyceride and 43.5 +/- 9.56 mg dL(-1) urea. The following spermatological parameters were found: sperm volume 14.44 +/- 1.16 mL, sperm motility 80.60 +/- 1.55%, movement duration 67.68 +/- 4.32 s, density 15.43 +/- 0.72 x 10(9) mL(-1), total density 337.43 + 45.86 x 10(9) and pH 7.24 +/- 0.17. The Na(+) and Ca(2+) ions correlated negatively with spermatozoa motility (r = -0.453, P > 0.05 and r = -0.192, P > 0.05) respectively. The K(+) ion correlated positively with spermatozoa motility (r = 0.545, P > 0.05). But a statistically significant correlation was not observed between sperm motility and seminal plasma parameters. The following correlations were observed between mineral and organic components. The Mg(2+) was positively correlated with glucose and cholesterol (r = 0.692, P < 0.05 and r = 0.680, P < 0.05) respectively. A highly significant positive relationship was also found between Mg(2+) and total protein (r = 0.837, P < 0.01). On the other hand, a significantly negative relationship was found between Ca(2+) and triglyceride (r = -0.639, P < 0.05). These parameters should be considered when developing procedures for either artificial fertilization or for cryopreservation of grass carp sperm.