The present study intended to assess students' knowledge on an ecology test which consists of two parts, multiple choice and open ended section. It also aimed to find out the effect of school, gender and grade level on the student performances in the test. This study employed a survey approach. The sample was consisted of 212 seventh and eighth grade students attending four elementary schools in Kirikkale city, Turkey. In this study, a previous data (Oner Arma an, 2006) were re-analysed. The analyses of independent T-test, Mann-Whitney U test, and ANOVA with SPSS revealed that there were some differences between schools and gender groups on the parts of the test. On the other hand no differences were found within grade levels. When the multiple-choice section of the test was considered, the mean scores of the male students were significantly higher than the females; in open-ended section of the test the mean scores of the female students were significantly higher than their counterparts. (C) 2010 Published by Elsevier Ltd.