In the study, new nanospheres support containing ferrocene were synthesized and characterized, then the properties of nanospheres as biocatalysis were researched. Novel support was prepared from (Aminomethyl) polystyrene (AMP), tryptophan (Trp) and ferrocene aldehyde (Fc) and the enzymatic properties of immobilized glucose oxidase enzyme (GOx) were investigated. Modified polystyrene was characterized IR spectra, Gel permeation chromatography and Scanning electron microscopy. Ferrocene-tagged nanospheres-GOx shows high reusability and storage capacity and fast incubation time determination of glucose. After 12 months, immobilized GOx to the AMP-Trp and AMP-Trp-Fc retained ca 20 % and 40 % for their original activity, respectively. High activities were found for modified polymers AMP-Trp-Fc retains more than 35 % of the initial activity after fifteenth successive cycles, which is a perfect performance.