The cytological features including chromosome number, karyotypic characteristics, C-banding and silver NOR-banding were investigated in a local cultivar of Allium sativum L., from Turkey. Actual lengths, relative lengths, L/S arm ratios of mitotic chromosomes were calculated from best six metaphase plates. Only Chromosome 5 was submedian, all the others were median. C-banding was observed on 4 chromosomes. Bands were centromeric on Chromosome 1 and 4, located to the neighbourhood of the secondary constrictions on Chromosome 5 and were interstitial close to centromere on Chromosome 8. Darkly stained silver nitrate bandings were present in the NORs of Chromosome 5 and 7, on Chromosome 1 and 8 in some cells.