Detection of the cry1 gene in Bacillus thuringiensis isolates from agricultural fields and their bioactivity against two stored product moth larvae


Bozlagan I., AYVAZ A., Ozturk F., AÇIK L. , AKBULUT M., YILMAZ S.

TURKISH JOURNAL OF AGRICULTURE AND FORESTRY, cilt.34, sa.2, ss.145-154, 2010 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 34 Konu: 2
  • Basım Tarihi: 2010
  • Doi Numarası: 10.3906/tar-0905-19
  • Dergi Adı: TURKISH JOURNAL OF AGRICULTURE AND FORESTRY
  • Sayfa Sayıları: ss.145-154

Özet

Sixty Bacillus spp. isolates were obtained from different agricultural fields in Kayseri province using selective media. Total DNA was isolated and analyzed by PCR using cry1 general primers. Of the 60 tested isolates, 17 carried the cry) gene. B. thuringiensis subsp. kurstaki and all other isolates exhibited approximately 19-kb plasmid bands.