Detection of the cry1 gene in Bacillus thuringiensis isolates from agricultural fields and their bioactivity against two stored product moth larvae

Bozlagan I., AYVAZ A., Ozturk F., AÇIK L., AKBULUT M., YILMAZ S.

TURKISH JOURNAL OF AGRICULTURE AND FORESTRY, vol.34, no.2, pp.145-154, 2010 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 34 Issue: 2
  • Publication Date: 2010
  • Doi Number: 10.3906/tar-0905-19
  • Journal Name: TURKISH JOURNAL OF AGRICULTURE AND FORESTRY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.145-154
  • Keywords: Bacillus thuringiensis, cry1 primer, Ephestia kuehniella, Plodia interpunctella, STRAINS, IDENTIFICATION, SOIL, LEPIDOPTERA, HABITATS
  • Gazi University Affiliated: Yes

Abstract

Sixty Bacillus spp. isolates were obtained from different agricultural fields in Kayseri province using selective media. Total DNA was isolated and analyzed by PCR using cry1 general primers. Of the 60 tested isolates, 17 carried the cry) gene. B. thuringiensis subsp. kurstaki and all other isolates exhibited approximately 19-kb plasmid bands.