Dose-dependent immunohistochemical and ultrastructural changes after oral methylphenidate administration in rat heart tissue

Take G., Bahçelioğlu M. , Oktem H., Tunc E., Gozil R., Erdogan D., ...Daha Fazla

Journal of Veterinary Medicine Series C: Anatomia Histologia Embryologia, cilt.37, ss.303-308, 2008 (SCI Expanded İndekslerine Giren Dergi) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 37
  • Basım Tarihi: 2008
  • Doi Numarası: 10.1111/j.1439-0264.2008.00845.x
  • Dergi Adı: Journal of Veterinary Medicine Series C: Anatomia Histologia Embryologia
  • Sayfa Sayıları: ss.303-308


Methylphenidate, more commonly known as Ritalin, is a piperidine derivative and is the drug most often used to treat attention deficit/hyperactivity disorder, one of the most common behavioural disorders of children and young adults. Our aims were to investigate dose-dependent immunohistochemical D2 expression and ultrastructural changes of the rat heart tissue, and to demonstrate possible toxicity of the long-term and high dose use of the methylphenidate. In this study, 27 female pre-pubertal Wistar albino rats, divided into three different dose groups (5, 10 and 20 mg/kg) and their control groups, were used. They were treated orally with methylphenidate dissolved in saline solution for 5 days/week during 3 months. At the end of the third month, after perfusion fixation, left ventricle of cardiac tissue was removed. Paraffin, semi-thin and thin sections were collected and immunohistochemical, terminal deoxynucleotidyl transferase-mediated Dig-dUTP nick end labelling assay and ultrastructural studies were performed. In conclusion, we believe that Ritalin is dose-related affecting dopaminergic system to increase heart rhythm and contraction. Thus, this drug may cause degenerative ultrastructural changes in mitochondrial path. © 2008 The Authors.