Immobilization of Trametes versicolor laccase on chitosan/halloysite as a biocatalyst in the Remazol Red RR dye.


Hürmüzlü R., Okur M., Saraçoğlu N.

International journal of biological macromolecules, vol.192, pp.331-341, 2021 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 192
  • Publication Date: 2021
  • Doi Number: 10.1016/j.ijbiomac.2021.09.213
  • Journal Name: International journal of biological macromolecules
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, EMBASE, Food Science & Technology Abstracts, INSPEC, MEDLINE, Veterinary Science Database
  • Page Numbers: pp.331-341
  • Keywords: Laccase, Chitosan, Halloysite, Immobilization, Dye removal, STATISTICAL OPTIMIZATION, HALLOYSITE NANOTUBES, CHITOSAN BEADS, TEXTILE DYES, AZO-DYE, DECOLORIZATION, REMOVAL, BIONANOCOMPOSITES, BIODEGRADATION, DEGRADATION
  • Gazi University Affiliated: Yes

Abstract

In this study, the laccase obtained from Trametes versicolor was immobilized onto the chitosan(CTS)/halloysite (HNT) beads. In the immobilization step, the effects of chitosan (1-3% w/v), halloysite (0-2% w/v), glutaraldehyde (0.5-1.5% v/v) and enzyme concentrations (1-3%) on loading and immobilization efficiency were investigated. SEM, FT-IR, XRD, TGA and XPS analyses were performed to examine the structure of beads. In addition, the effects of parameters such as pH (4-10), temperature (25-55 degrees C), storage life on the activity of free and immobilized laccase were also investigated. The activities of free and immobilized laccase preserved 23% and 56% of its initial activity at the end of 59 days of storage. The effects of mediators such as 2.2-azino-bis(3ethylbenzothiazoline-6-sulfonic acid (ABTS), 1-Hydroxybenzotriazole hydrate (HBT), 2,2,6,6-Tetramethyl-1piperidinyloxy (TEMPO) and violuric acid (VLA) on the dye removal efficiency were investigated. Reusability of the CTS/HNT/Lac in the presence of HBT and VLA mediators, which enable the highest dye removal, was tested. After 15 cycles, 42% and 54% dye removal were achieved with the CTS/HNT/Lac in the medium containing HBT and VLA, and 42% and 49% of the activity is preserved, respectively. This study showed that CTS/ HNT/Lac can be used repeatedly for Remazol Red RR dye removal.