Detection of enteric parasites and molecular characterization of Giardia duodenalis and Blastocystis sp. in patients admitted to hospital in Ankara, Turkey

Sarzhanov F., Koster P. C., DOĞRUMAN AL F., Bailo B., Dashti A., Demirel-Kaya F., ...Daha Fazla

PARASITOLOGY, cilt.148, sa.5, ss.550-561, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 148 Sayı: 5
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1017/s0031182020001821
  • Dergi Adı: PARASITOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Agricultural & Environmental Science Database, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, EMBASE, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.550-561
  • Anahtar Kelimeler: Blastocystis, Cryptosporidium, Dientamoeba fragilis, Entamoeba histolytica, Giardia duodenalis, intestinal parasites, molecular epidemiology, multilocus genotyping, Turkey, REAL-TIME PCR, DIENTAMOEBA-FRAGILIS, ZOONOTIC TRANSMISSION, INTESTINAL PARASITES, DEVELOPING-COUNTRIES, DIARRHEAL DISEASE, STOOL SAMPLES, CHILDREN, PREVALENCE, CRYPTOSPORIDIUM
  • Gazi Üniversitesi Adresli: Evet

Özet

This epidemiological study assesses the occurrence of enteric parasites in 4303 patients attended at two public hospitals in Ankara (Turkey) during 2018-2019. Microscopy was used as a screening test. Giardia duodenalis was also identified using a commercial ELISA for the detection of parasite-specific coproantigens. Giardia-positive samples by microscopy/ELISA were confirmed by real-time PCR and characterized using a multilocus genotyping scheme. Blastocystis sp. was genotyped in a sample subset. Blastocystis sp. (11.1%, 95% CI 11.4-14.8%) and G. duodenalis (1.56%, 95% CI 1.22-1.96) were the most prevalent pathogens found. Cryptosporidium spp., Entamoeba histolytica and intestinal helminths were only sporadically (<0.5%) found. For G. duodenalis, sequence (n = 30) analyses revealed the presence of sub-assemblages AII (23.3%), discordant AII/AIII (23.3%) and mixed AII + AIII (6.7%) within assemblage A, and BIII (10.0%), BIV (3.3%) and discordant BIII/BIV (23.3%) within assemblage B. Two additional sequences (6.7%) were assigned to the latter assemblage but sub-assemblage information was unknown. No associations between G. duodenalis assemblages/sub-assemblages and sociodemographic and clinical variables could be demonstrated. For Blastocystis sp., sequence (n = 6) analyses identified subtypes ST1, ST2 and ST3 at equal proportions. This is the first molecular characterization of G. duodenalis based on MLG conducted in Turkey to date.