Quantitative determination of ambroxol in tablets by derivative UV spectrophotometric method and HPLC

Dincer Z., Basan H., Goger N.

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, cilt.31, sa.5, ss.867-872, 2003 (SCI-Expanded) identifier identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 31 Sayı: 5
  • Basım Tarihi: 2003
  • Doi Numarası: 10.1016/s0731-7085(02)00664-7
  • Dergi Adı: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.867-872
  • Anahtar Kelimeler: ambroxol, derivative UV spectrophotometry, reversed-phase high-performance liquid chromatography, HYDROCHLORIDE, CHROMATOGRAPHY, FLUIDS
  • Gazi Üniversitesi Adresli: Evet

Özet

A derivative UV spectrophotometric method for the determination of ambroxol in tablets was developed. Determination of ambroxol in tablets was conducted by using first-order derivative UV spectrophotometric method at 255 nm (n = 5). Standards for the calibration graph ranging from 5.0 to 35.0 mug/ml ere prepared from stock solution. The proposed method was accurate with 98.6 +/- 0.4%, recovery value and precise with coefficient of variation (CV) of 1.22. These results were compared with those obtained by reference methods, zero-order UV spectrophotometric method and reversed-phase high-performance liquid chromatography (HPLC) method. A reversed-phase C-18 column with aqueous phosphate (0.01 M)-acetonitrile-glacial acetic acid (59:40:1, v/v/v) (pH 3.12) mobile phase was used and UV detector was set to 252 nm. Calibration solutions used in HPLC were ranging from 5.0 to 20.0 mug/ml. Results obtained by derivative UV spectrophotometric method was comparable to those obtained by reference methods, zero-order UV spectrophotometric method and HPLC, as far as ANOVA test, F-calculated = 0.762 and F-theoretical = 3.89, was concerned, (C) 2003 Elsevier Science B.V. All rights reserved.