A Rapid, Precise, and Sensitive LC-MS/MS Method for the Quantitative Determination of Urinary Dopamine Levels via a Simple Liquid-liquid Extraction Technique Basit Sıvı-sıvı Ekstraksiyon Tekniği ile İdrar Dopamin Düzeylerinin Kantitatif Tayini için Hızlı, Kesin ve Hassas Bir LC-MS/MS Yöntemi
Turkish Journal of Pharmaceutical Sciences, cilt.18, sa.6, ss.761-769, 2021 (ESCI, Scopus, TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 18 Sayı: 6
- Basım Tarihi: 2021
- Doi Numarası: 10.4274/tjps.galenos.2021.60486
- Dergi Adı: Turkish Journal of Pharmaceutical Sciences
- Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI), Scopus, Academic Search Premier, EMBASE, International Pharmaceutical Abstracts, TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.761-769
- Anahtar Kelimeler: Dopamine, LC-MS, MS, urine, method validation, SOLID-PHASE EXTRACTION, MASS-SPECTROMETRIC ASSAY, MONOAMINE NEUROTRANSMITTERS, BIOGENIC-AMINES, HUMAN PLASMA, ACETIC-ACID, CHROMATOGRAPHY, CATECHOLAMINES, ONLINE, METANEPHRINES
- Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
- Gazi Üniversitesi Adresli: Evet
Özet
© Turk J Pharm Sci, Published by Galenos Publishing House.Objectives: Dopamine (DA) is a prominent biochemically complex neurotransmitter and immunomodulator. The quantification of DA could contribute to a better understanding of how endocrine system, cardiovascular and renal functions are regulated. The study aims to develop a rapid, precise, and extremely sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for routine clinical quantification of DA in urine. Materials and Methods: Urine samples were extracted via one simple and rapid liquid-liquid extraction technique; then analyzed using a sensitive LC-MS/MS method developed by multiple reaction monitoring mode. Results: DA and internal standard (IS) retention durations were found to be 2.28 min and 2.24 min, respectively. The mean extraction recovery of DA and DA-IS in urine was above 95.62%. DA calibration curve in urine was linear (r2≥0.998) ranging from 20 ng/mL to 1000 ng/mL. The maximum intra-day and inter-day precisions were 5.87 and 2.81, respectively and coefficients of variation were 10.55% and 7.57%, respectively. Conclusion: A rapid, precise, sensitive and quantitative LC-MS/MS detection of DA without the use of derivatization, evaporation, reconstitution and ion-pairing reagents has been developed with a simple and non-invasive sample technique for clinical laboratory applications, basic neuroscience research and drug development studies.