Karyotype and C-banding analyse have been performed to Sternbergia lutea and S. sicula from Turkey. While S. lutea was found to be diploid (2n = 22) and triploid (2n = 33), S. sicula proved to be diploid with 2n = 22. Chromosome lengths in S. lutea and S. sicula are 19.85-5.15 μm and 17.88-5.16 μm, respectively. While all chromosomes in S. lutea showed centromeric C-band, in S. sicula, eight chromosomes carry constitutive heterochromatin, being centromeric on seven and interstitial on one. Three chromosomes, 4, 7 and 10, in S. sicula, show no C-band. Based on these results, the relationships between the two taxa are discussed.