Functional polymeric supports for immobilization of cholesterol oxidase


Akkaya B., Sahin F., DEMİREL G. , TÜMTÜRK H.

BIOCHEMICAL ENGINEERING JOURNAL, cilt.43, sa.3, ss.333-337, 2009 (SCI İndekslerine Giren Dergi) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 43 Konu: 3
  • Basım Tarihi: 2009
  • Doi Numarası: 10.1016/j.bej.2008.11.003
  • Dergi Adı: BIOCHEMICAL ENGINEERING JOURNAL
  • Sayfa Sayıları: ss.333-337

Özet

Here, we have reported the useful functional polymeric Supports for possible application of enzyme immobilization. Functional polymers were prepared by free radical polymerization from different monomers (i.e., methylmetacrylate, glycidylmethacrylate, acrylamide, etc.) and N,N-methylenebis(acrylamide) (MBAAm) crosslinker. Cholesterol oxidase (ChOx) [EC.1.1.3.6] was then covalently immobilized onto these functional supports via epichlorohydrin (ECH) and carbodiimide (EDAC) as the activating agents. It was observed that, after 60th use in 5 days, the retained activities for immobilized enzymes onto poly(methyl methacrylate-co-glycidyl methacrylate) [P(MMA-co-GMA)] and poly(acrylamide-co-acrylic acid)/polyethyleneimine [P(AAm-co-AA)/PEI] supports were found as 56% and 83%, respectively. (C) 2008 Published by Elsevier B.V.