Use of alkaline Comet assay (single cell gel electrophoresis technique) to detect DNA damages in lymphocytes of operating room personnel occupationally exposed to anaesthetic gases

Sardas, S; Aygun, N; Gamli, M; Unal, Y; Berk, N; Karakaya, AE

doi:10.1016/s1383-5718(98)00113-2

Use of alkaline Comet assay (single cell gel electrophoresis technique) to detect DNA damages in lymphocytes of operating room personnel occupationally exposed to anaesthetic gases

Atıf İçin Kopyala

Sardas S., Aygun N., Gamli M., Unal Y., Berk N., Karakaya A.

MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, cilt.418, ss.93-100, 1998 (SCI-Expanded)

Yayın Türü: Makale / Tam Makale
Cilt numarası: 418
Basım Tarihi: 1998
Doi Numarası: 10.1016/s1383-5718(98)00113-2
Dergi Adı: MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
Sayfa Sayıları: ss.93-100
Gazi Üniversitesi Adresli: Hayır

Özet

Here, we report the possible in vivo induction DNA damage by exposure to various waste anaesthetic gases such as halothane, nitrous oxide and isoflurane. The alkaline comet assay (single cell gel electrophoresis technique) was carried out on 66 operating room personnel (anaesthetists [doctors]; anaesthesia nurses and anaesthesia unit technicians) currently employed at the Ankara Hospital in Turkey. A significant increase in the number of lymphocytes with DNA migration was observed in operating room personnel as compared to controls. Also, the extent of damage in exposed smokers were significantly higher than exposed nonsmokers. This study supports the existence of an association between DNA damage and occupational exposure to inhalation anaesthetics. (C) 1998 Elsevier Science B.V. All rights reserved.