Akademik Veri Yönetim Sistemi
BIOSENSORS 2025 35th Anniversary World Congress on Biosensors, Lisbon, Portekiz, 19 - 22 Mayıs 2025, ss.1, (Özet Bildiri)
The monitoring of bacteria plays
an important role in areas such as food and drinking water safety and the
diagnosis of infectious diseases. Although bacteria can be detected by various
methods, culture methods are generally used for accurate, reproducible, and
selective bacterial identification. However, the culture method is
time-consuming and requires trained personnel. One alternative to the culture
method is PCR, but molecular detection methods like PCR require a DNA
extraction step beforehand. During DNA extraction, DNA is concentrated and
purified. We propose a DNA extraction platform by integrating magnetic beads
with a microfluidic platform produced using Stereolithography (SLA), which
requires minimal solution volume, and is easy and fast to use. To produce a 3D
object with SLA, liquid polymers are cured under UV light. This technique does
not require a clean room, mask-mold, or experienced personnel during
production, making it cheaper compared to other methods. The SLA-produced
microfluidic platform is designed with two inlets, a mixing channel, and a
well. One inlet is used for bacterial entry, while the other is for DNA
extraction solutions and magnetic beads. DNA released by the lysis of Staphylococcus
aureus (S. aureus) mixed in the channel is collected in the well, and DNA
held by magnetic beads is purified with washing solutions. The purified and
concentrated DNA is then taken from the well and analyzed with qPCR. The qPCR
results demonstrate successful DNA extraction from the SLA-produced
microfluidic platform. This platform enables fast, easy DNA extraction from low
sample volumes, using minimal solution amounts and without requiring
centrifugation.