Akademik Veri Yönetim Sistemi
Turkish Journal of Biochemistry, cilt.48, sa.2, ss.73-86, 2024 (SCI-Expanded)
Objectives: Pesticides, widely used for pest control in agriculture, public health and veterinary medicine, are usually present as pollutants in aquatic environments. After contamination of water bodies, pesticides cause negative effects on the non target organisms and long term problems in the ecosystem. Lambda-cyhalothrin (LCH) is a chemical compound, which belongs to the family of synthetic pyrethroids (type II), used as an active ingredient in several insecticides. In this study, we investigated the toxic effects of LCH exposure for 24 and 72 h on zebrafish as a DNA damage. Materials-Methods: The zebrafish (Danio rerio) was obtained from commercial suppliers in Türkiye. Experiments were carried out in Gazi University Department of Biology Education. Zebrafish with an average length of 2.59±0.21 cm were placed in 3 aquariums of 10 liters filled with spring water and adapted to the laboratory conditions for 15 days. LCH was prepared at concentrations of 0.1 and 1 µg/L and applied for 24-72 h. After the exposure period, whole body zebrafish was prepared for 8-OHdG analysis. 8-OHdG measurement was done by the Agilent LC-MS/MS device using the electrospray ionization method and positive ion mode. Results: In our study, it was observed that the whole-body tissue’ 8-OHdG values were significantly increased in the group exposed to LCH for 72 hours (9.82±1.44 ng/100 g doku) compared to the control group (6.60±1.78 ng/100 g doku, p=0.004). These results suggest that LCH could lead oxidative DNA damage by causing an increase 8-OhdG activities. Conclusions: These results suggested that LCH exposure had effects on DNA damage (8-OHdG) of the zebrafish. Evaluating the toxic effects of an individual pesticide may not be enough for protecting ecological environment due to interactions among substances The data acquired from this study provided valuable insights into the toxicity of LCH to zebrafish.