A differential pulse polarographic method was described for the determination of rutin that is obtained from Cruciata taurica (Pallas ex willd.) Ehrend s.I. Differential pulse polarograms of rutin show a distinct peak at -1.83 V (vs Ag, AgCl) at pH 6.00 Sorensen buffer. The total amount of flavonoids in Cruciata taurica (Pallas ex Wild) Ehrend.s.I. has been found to be 0.45% by using rutin as a standard.