Akademik Veri Yönetim Sistemi
Ankara Universitesi Eczacilik Fakultesi Dergisi, cilt.47, sa.2, 2023 (Scopus)
Objective: Diabetes and obesity are among the most common health problems today. New drug sources are needed to treat these problems. Hypericum scabrum L., which is known for its traditional use among the public, has been the subject of this research. Within the scope of the research, it was aimed to investigate the antioxidant, antidiabetic, anti-obesity, anticholesterol activities, and phytochemical content of ethanol (80%) extract prepared from the aerial parts of the H. scabrum. Material and Method: The total phenol, total flavonoid contents, and phytochemical content of the ethanol (80%) extract prepared from the aerial parts of the H. scabrum were evaluated. Additionally, the phenolic and flavonoid compounds in the extract were investigated with reverse phase HPLC and their quantitative analysis was carried out. The antioxidant effect of the extract was evaluated with DPPH radical-scavenging effect, metal chelating capacity, ferric reducing power, ABTS radical scavenging effect, and total antioxidant capacity tests. In the investigation of the antidiabetic effect of the extract, α-glucosidase and α-amylase enzyme inhibitory activities were investigated. To investigate the effects of anti-obesity and lipid metabolism, pancreatic lipase enzyme inhibitory activity and cholesterol esterase enzyme inhibitory activity were investigated, respectively. Result and Discussion: The extract showed 90.16 ± 0.13% DPPH radical scavenging and 97.53 ± 0.22% ABTS radical scavenging activity at 2 mg/ml concentration. The extract had an inhibitory activity of 98.80 ± 0.51 % on the α-glucosidase enzyme at a concentration of 2 mg/ml, and this value was close to the acarbose result used as the standard substance (99.13 ± 0.11%). The extract showed inhibitory activity of 68.63 ± 1.05% on the cholesterol esterase enzyme at a concentration of 2 mg/ml. According to the results of reversed-phase HPLC analysis, it was found that the extract contained 1.047 ± 0.000 g/100 g extract chlorogenic acid. According to the results, it was concluded that the extract may be a natural source with antidiabetic effects and further studies are needed to investigate this effect.