The antibacterial and antifungal activities of various extracts from the herbs of Cirsium hypoleucum were screened by microdilution method against both the standard and the isolated strains of Escherichia coli, Pseudomonas aeroginosa, Enterococcus faecalis, Staphylococcus aureus, and the fungi Candida albicans and Candida parapsilosis. Additionally, both Herpes simplex (HSV) and Parainfluenza viruses (PIV) were used for the determination of antiviral activity of the herb extracts of this plant by using Vero cell line. Ampicillin, ofloxacin, ketoconazole, fluconazole, acyclovir and oseltamivir were used as the control agents. The data obtained from antiviral activity screening showed that remaining water fraction had significant activity against HSV. Additionally, methanol extract, ethylacetate and n-butanol fractions displayed remarkable antibacterial activity against isolated strains of S. aureus at 32 mu g/ml concentration. However, no significant activity was observed with any of the extracts obtained from antifungal and antibacterial activity.