Immobilization of laccase onto spacer-arm attached non-porous poly(GMA/EGDMA) beads: Application for textile dye degradation

Arica M. Y., Altintas B., Bayramoglu G.

BIORESOURCE TECHNOLOGY, cilt.100, sa.2, ss.665-669, 2009 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 100 Sayı: 2
  • Basım Tarihi: 2009
  • Doi Numarası: 10.1016/j.biortech.2008.07.038
  • Dergi Adı: BIORESOURCE TECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.665-669
  • Anahtar Kelimeler: Laccase, Immobilized enzyme, Enzyme technology, Enzyme reactor, Dye removal, COVALENT IMMOBILIZATION, GLYCIDYL METHACRYLATE, CATALYTIC-PROPERTIES, POLYPHENOL OXIDASE, REACTIVE MEMBRANES, MAGNETIC BEADS, STABILITY, POLYMERIZATION, SUPPORTS, TYROSINASE
  • Gazi Üniversitesi Adresli: Evet

Özet

Non-porous poly(glycidyl methacrylate/ethyleneglycol dimetacrylate) (poly(GMA/EGDMA)) beads were prepared by suspension polymerization. The enzyme (i.e. laccase) was covalently immobilized onto plain and spacer-arm attached poly(GMA/EGDMA) beads. The amount of immobilized enzyme on the plain and spacer-arm attached beads was determined as 5.6 and 4.9 mg/g, respectively. The maximum activity (V-max) and Michaelis constant (K-m) of laccase immobilized on the spacer-arm attached beads, were found to be 77.6 U/min and 0.47 mM, respectively. Finally, the immobilized laccase was operated in a batch system, and textile dye Reactive Red 120 was successfully decolorized in the enzyme reactor. (c) 2008 Elsevier Ltd. All rights reserved.