Immobilization of laccase onto spacer-arm attached non-porous poly(GMA/EGDMA) beads: Application for textile dye degradation

Arica M. Y. , Altintas B., Bayramoglu G.

BIORESOURCE TECHNOLOGY, cilt.100, sa.2, ss.665-669, 2009 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 100 Konu: 2
  • Basım Tarihi: 2009
  • Doi Numarası: 10.1016/j.biortech.2008.07.038
  • Sayfa Sayıları: ss.665-669


Non-porous poly(glycidyl methacrylate/ethyleneglycol dimetacrylate) (poly(GMA/EGDMA)) beads were prepared by suspension polymerization. The enzyme (i.e. laccase) was covalently immobilized onto plain and spacer-arm attached poly(GMA/EGDMA) beads. The amount of immobilized enzyme on the plain and spacer-arm attached beads was determined as 5.6 and 4.9 mg/g, respectively. The maximum activity (V-max) and Michaelis constant (K-m) of laccase immobilized on the spacer-arm attached beads, were found to be 77.6 U/min and 0.47 mM, respectively. Finally, the immobilized laccase was operated in a batch system, and textile dye Reactive Red 120 was successfully decolorized in the enzyme reactor. (c) 2008 Elsevier Ltd. All rights reserved.