Aim The relationship between body fluid composition measurements and echocardiographic tissue Doppler measurements before and after hemodialysis (HD) in end-stage renal disease is investigated. Materials and methods BCM (BCM-Fresenius Medical Care) Body Composition Monitor was used to measure the fluid status and body composition of patients by BIA method. TDE was performed before and after HD and the measurements were recorded. Results The mean age of the 72 patients was 53.7 +/- 17.2 years. There was a significant decrease in over hydration (OH) status, total body fluid (TBW), extracellular body fluid (ECW), intracellular body fluid (ICW), ECW/ICW ratio in patients after HD (p < 0.001, p0.001, p0.001, p0,001). A significant decrease in lateral, septal and tricuspid E' measurements and in E'/A' ratio was seen after HD (p: 0.012, p: 0.001,p < 0.001,p: 0.002, p0.001, p0,001). There was a significant difference in A' measurements from septal annulus in patients before and after HD (p: 0.001). A significant increase was observed in isovolemic contraction volume (IVCV) measurements from lateral, septal and tricuspid annulus in patients after HD (p < 0.001, p0.001, p: 0.012). A correlation was determined between the differences of lateral and tricuspid valve E' in patients before and after HD and the differences of ECW in patients before and after HD (p: 0.007r: 0.401,p: 0.017r: 0.358). Conclusion In this study; a correlation was determined between the acute decrease in OH amount and alteration in echocardiographic tissue Doppler parameters in patients after HD.