Akademik Veri Yönetim Sistemi
KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI, cilt.17, sa.2, ss.309-314, 2011 (SCI-Expanded)
We aimed to investigate the possible neurotoxic effects of single and repeated doses of diclofenac sodium administered to rats. The current study included 24 male Sprague-Dawley rats weighing 250-300 g. At the end of a 4-h fasting period, the rats were randomly split into 3 groups following the establishment of anesthesia with intraperitoneal delivery of 100 mg kg(-1) of ketamine hydrochloride and 10 mg kg(-1) of xylazine chloride. Group 2 (n=8) received 10 mu l (200 mu g) of intrathecal diclofenac sodium on 7th day as a single dose, whereas the rats in group 1 (n=8) and group 3 (n=8) were received 10 mu l of intrathecal 0.9% saline and 10 mu l (200 mu g) of intrathecal diclofenac sodium via a 0.40 x 50 mm needle per day for 7 days. During the course of the study the animals were examined with regard to clinical toxicity. After fixating the tissues by injection of 10% formaldehyde, spinal cords were explored and evaluated histopathologically with electron microscopy. Statistical analysis was performed with the Kruskal Wallis test. P values < 0.05 were considered statistically significant. While electron microscopic examination showed no changes in the control group, diclofenac sodium exhibited neurotoxic effects that were more marked following the 7-days treatment. Diclofenac sodium was neurodegenerative, depending on the dose, and cellular organelles were observed to have compression associated with extracellular edema. Neurodegeneration was thought to be occured with a significant reduction in cellular activity.