Development of a new real-time PCR screening kit for HbS and common beta-thalassemia mutations observed in Turkey


Kan Karaer D., ERGÜN M. A. , ILGIN RUHİ H., Ozturk J., Kara H., Reisoglu Cakmak D., ...More

TURKISH JOURNAL OF MEDICAL SCIENCES, vol.47, no.3, pp.973-978, 2017 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 47 Issue: 3
  • Publication Date: 2017
  • Doi Number: 10.3906/sag-1606-49
  • Title of Journal : TURKISH JOURNAL OF MEDICAL SCIENCES
  • Page Numbers: pp.973-978

Abstract

Background/aim: IVSI-110 (G> A), IVSI-6 (T> C), IVSII-1 (G> A), IVSII-745 (C> G), IVSI-1 (G> A), and HbS are mutations covering 76% of all the beta-globin mutations in the Turkish population. In this study, our aim is to develop a reliable, fast, real-time kit for these mutations using the TaqMan probe method.