In this study, the production of exopolysaccharides (EPS) by Pseudomonas aeruginosa G I and Pseudomonas putida G 12 strains on Pseudomonas Agar P Medium with 2% glycerol was investigated. EPS yields from these strains were detected (192 mg/L, 182 mg/L, respectively). Pseudomonas Agar P Medium with 2% glycerol (w/v) was used as control. Also, EPS productions of these Pseudomonas strains have been determined in sugarbeet molasses [2-6% w/v concentrations (2-6 molasses/100 mL water)] as a complex carbon source. The highest EPS production of the G1 strain (397 mg/L) was found in 6% w/v molasses concentration according to the control, while the highest EPS production of the G12 strain (367 mg/L) was found in 4% w/v molasses concentration according to the control. The use of sugarbeet molasses as an inexpensive alternative to glycerol was efficient.