Development of an acetylcholinesterase-choline oxidase based biosensor for acetylcholine determination

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Tunc A. T., Koyuncu E. A., ARSLAN F.

ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY, vol.44, no.7, pp.1659-1664, 2016 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 44 Issue: 7
  • Publication Date: 2016
  • Doi Number: 10.3109/21691401.2015.1080167
  • Journal Name: ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1659-1664
  • Keywords: acetylcholine, acetylcholinesterase, biosensor, choline oxidase, polyaniline, polyvinylsulphonate, AMPEROMETRIC BIOSENSOR, POLYVINYLSULFONATE FILM, DIOXIDE COMPOSITE, POLYPYRROLE FILM, ELECTRODE, IMMOBILIZATION, POLYANILINE, MEMBRANE, URICASE, DESIGN
  • Gazi University Affiliated: Yes

Abstract

In this article, a novel amperometric acetylcholine (ACh) biosensor with immobilization of acetylcholinesterase-choline oxidase (AChE-ChO) on electrochemically polymerized polyaniline-polyvinylsulphonate (PANI-PVS) film has been successfully developed for the first time. AChE and ChO enzymes were immobilized on PANI-PVS film by cross-linking with using glutaraldehyde (GA) and bovine serum albumin (BSA). Determination of ACh was carried out by oxidation of enzymatically produced H2O2 at +0.4 V versus Ag/AgCl. Optimum pH and temperature were found to be 8.0 and 60 degrees C, respectively. The linear part was in the region 1.0x10(-7)-6.0x10(-7)M (R-2=0.990). Storage stability and operation stability of the enzyme electrode were also studied.