Effect of topical vitamin E on ethanol-induced corneal epithelial apoptosis
JOURNAL OF REFRACTIVE SURGERY, cilt.21, sa.6, ss.761-763, 2005 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 21 Sayı: 6
- Basım Tarihi: 2005
- Doi Numarası: 10.3928/1081-597x-20051101-19
- Dergi Adı: JOURNAL OF REFRACTIVE SURGERY
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.761-763
- Gazi Üniversitesi Adresli: Evet
Özet
PURPOSE: Ethanol is used to loosen the corneal epithelium before photoablation in laser subepithelial keratomileusis (LASEK). In this study, the apoptotic index of corneal epithelium after ethanol exposure and the effects of topical vitamin E were evaluated. METHODS: The study was performed on 28 rabbit eyes in four groups. Group 1 comprised the controls. In group 2, 20% ethanol was applied topically for 20 seconds. In group 3, topical vitamin E was applied following 20% ethanol application. In group 4, only topical vitamin E was applied. Apoptosis was evaluated with TUNEL assay and transmission electron microscopy. RESULTS: Epithelial apoptosis was detected in all specimens in group 2. No apoptosis was detected in other groups except for one eye in group 1. The apoptotic index in group 2 was statistically higher than other groups (P<.001). CONCLUSIONS: Topical vitamin E can prevent ethanol-induced apoptosis in the corneal epithelium in rabbits.