Nitric oxide, produced in many mammalian tissues, is well known as an important mediator of many physiological and pathological responses. Nitric oxide synthesis plays an important role in many aspects of inflammation and wound healing. The purpose of this article is to comparatively investigate the effects of titanium, titanium + polyethylene glycol 4000, titanium + polyethylene glycol 4000 + N-nitro L-arginine methyl ester and intraperitoneally injected N-nitro-L-arginine methyl ester, on plasma nitric oxide, malondialdehyde and, heart tissue nitric oxide and malondialdehyde levels. Plasma malondialdehyde, Nitrite and Nitrate and heart tissue malondialdehyde, nitrite and nitrate levels decreased after i.p. N-nitro-L-arginine methyl ester administration (p < 0.05). This leads to the conclusion that N-nitro-L-arginine methyl ester inhibits the deteriorating effects of free radicals without affecting wound healing. N-nitro-L-arginine methyl ester in polyethylene glycol 4000 and titanium also have no effect on tissue and plasma nitrite and nitrate and malondialdehyde levels meaning that nitric oxide generation is not affected from titanium and local nitric oxide synthase inhibitor.