The new Schiff base 4-[(4-Hydroxy-3-fluoro-5-methoxy-benzylidene)amino]-1,5-dimethy1-2-phenyl-1,2-dihydro-pyrazol-3-one: Experimental, DFT calculational studies and in vitro antimicrobial activity

OCAK İSKELELİ N., ALPASLAN Y. B., DİREKEL Ş., ERTÜRK A. G., SÜLEYMANOĞLU N., Ustabas R.

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, cilt.139, ss.356-366, 2015 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 139
  • Basım Tarihi: 2015
  • Doi Numarası: 10.1016/j.saa.2014.12.071
  • Dergi Adı: SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.356-366
  • Anahtar Kelimeler: Schiff base, Crystal structure, FT-IR and NMR spectroscopy, Electronic absorption spectra, DFT calculations, Antibacterial and antifungal activity, DENSITY-FUNCTIONAL THEORY, EFFICIENT IMPLEMENTATION, ENERGIES, POTENTIALS, MOLECULES, CONTINUUM, MODEL
  • Gazi Üniversitesi Adresli: Evet

Özet

The synthesized Schiff base, 4-[(4-Hydroxy-3-fluoro-5-methoxy-benzylidene)amino1-1,5-dimethyl-2-phenyl-1,2-dihydro-pyrazol-3-one (I), has been characterized by C-13 NMR, H-1 NMR, 2D NMR (H-1-H-1 COSY and C-13 APT), FT-IR, UV-vis and X-ray single-crystal techniques. Molecular geometry of the compound I in the ground state, vibrational frequencies and chemical shift values have been calculated by using the density functional method (DFT) with 6-311++G(d,p) basis set. The obtained results indicate that optimized geometry can well reflect the crystal structural parameters. The differences between experimental and calculated results of FT-IR and NMR have supported the existence of intermolecular (O-H center dot center dot center dot O type) and intramolecular (C-H center dot center dot center dot O type) hydrogen bonds in the crystal structure. Molecular electrostatic potential (MEP), frontier molecular orbital analysis (HOMO-LUMO) and electronic absorption spectra were carried out at B3LYP/6-31 1G++(d,p). HOMO-LUMO electronic transition of 3.92 eV is due to contribution of the bands the n -> pi*. The antimicrobial activity of the compound I was determined against the selected 11 bacteria and 8 fungi by microdilution broth assay with Alamar Blue. In vitro studies showed that the compound I has no antifungal effect for selected fungal isolates. However, the compound I shows remarkable antibacterial effect for the bacteria; Streptococcus pneumoniae, Haemophilus influenzae and Enterococcus faecalis. (C) 2014 Elsevier B.V. All rights reserved.