Effects of microwave exposure and Gemcitabine treatment on apoptotic activity in Burkitt's lymphoma (Raji) cells


CANSEVEN KURŞUN A. G., EŞMEKAYA M. A., KAYHAN H., Tuysuz M. Z., Seyhan N.

ELECTROMAGNETIC BIOLOGY AND MEDICINE, cilt.34, sa.4, ss.322-326, 2015 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 34 Sayı: 4
  • Basım Tarihi: 2015
  • Doi Numarası: 10.3109/15368378.2014.919591
  • Dergi Adı: ELECTROMAGNETIC BIOLOGY AND MEDICINE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.322-326
  • Anahtar Kelimeler: Apoptosis, cell viability, non-ionizing, radiofrequency, PERIPHERAL-BLOOD LYMPHOCYTES, RADIOFREQUENCY RADIATION, BRAIN, EXPRESSION, FIELDS, GROWTH, WAVES
  • Gazi Üniversitesi Adresli: Evet

Özet

We investigated the effects of 1.8MHz Global System for Mobile Communications (GSM)-modulated microwave (MW) radiation on apoptotic level and cell viability of Burkitt's lymphoma (Raji) cells with or without Gemcitabine, which exhibits cell phase specificity, primarily killing cells undergoing DNA synthesis (S-phase). Raji cells were exposed to 1.8GHz GSM-modulated MW radiation at a specific absorption rate (SAR) of 0.350W/kg in a CO2 incubator. The duration of the exposure was 24h. The amount of apoptotic cells was analyzed using Annexin V-FITC and propidium iodide (PI) staining with flow cytometer. The apoptotic activity of MW exposed Raji cells was increased significantly. In addition, cell viability of exposed samples was significantly decreased. Combined exposure of MW and Gemcitabine increased the amount of apoptotic cells than MW radiation alone. Moreover, viability of MW+Gemcitabine exposed cells was lower than that of cells exposed only to MW. These results demonstrated that MW radiation exposure and Gemcitabine treatment have a synergistic effect on apoptotic activity of Raji cells.