Evaluation of hepatoprotective effect of Gentiana olivieri herbs on subacute administration and isolation of active principle

Orhan D., Aslan M., AKTAY G., Ergun E., Yesilada E., Ergun F.

LIFE SCIENCES, cilt.72, sa.20, ss.2273-2283, 2003 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 72 Sayı: 20
  • Basım Tarihi: 2003
  • Doi Numarası: 10.1016/s0024-3205(03)00117-6
  • Dergi Adı: LIFE SCIENCES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.2273-2283
  • Gazi Üniversitesi Adresli: Evet

Özet

Hepatoprotective effect of Gentiana olivieri Griseb. (Gentianaceae) flowering herbs on subacute administration were studied using in vivo models in rats. For the activity assessment on carbon tetrachloride-induced hepatic damage following biochemical parameters were evaluated; plasma and hepatic tissue malondialdehyde formation, and liver tissue glutathione level, as well as plasma transaminase enzyme levels (aspartate transferase and alanine transferase). Results of biochemical tests were also confirmed by histopathological examination. Through bioassay-guided fractionation procedures isoorientin, a known C-glycosylflavone, was isolated from the ethyl acetate fraction as the active antihepatotoxic constituent by silica gel column chromatography. Isoorientin exhibited significant hepatoprotective effect at 15 mg/kg b.w. dose. (C) 2003 Published by Elsevier Science Inc.