Akademik Veri Yönetim Sistemi
JOURNAL OF SOLID STATE ELECTROCHEMISTRY, cilt.25, sa.5, ss.1601-1610, 2021 (SCI-Expanded)
In this study, a new amperometric biosensor for the detection of glucose was developed. For this aim, the glassy carbon electrode surface was covered with a platinum nanoparticle graphene solution by dropping. The glucose oxidase enzyme was immobilized by cross-linking method on the modified electrode by graphene/platinum nanoparticles/Nafion (GR/PtNPs/NF) solution. Glucose determination was based on the oxidation of hydrogen peroxide at + 0.60 V produced by the enzymatic reaction on the glucose biosensor surface. Surface morphologies of film electrode and glucose biosensor were evaluated by atomic force microscopy (AFM), scanning electron microscopy (SEM), and surface contact angle analysis. The linear working range of biosensor for glucose was determined. The effects of temperature and pH on the response of the glucose biosensor were investigated. The K-m and V-max constants were calculated as 0.94 mM and 0.775 mu A min(-1), respectively. The determination limit for the glucose molecule of the prepared biosensor was found as 10 nM. Reproducibility and storage stability were determined for the glucose biosensor. The activity of glucose biosensor was maintained 94.5% at the end of 20 measurements. The effects of the interference occurring in biological environments on the response of the glucose biosensor were examined. The detection of glucose was carried out in synthetic blood sample by biosensor. Electrochemical studies show that graphene/platinum nanoparticles/Nafion (GR/PtNps/NF) film can present a promising biosensing platform and exhibits high sensitivity and selectivity for clinical diagnosis applications.