Ornithogalum ulophyllum Hand.-Mazz. with beautiful white flowers is an important medicinal and ornamental plant of the Middle Eastern countries and need exploitation for commercial propagation. The study reports in vitro mass proliferation of bulblets achieved from twin scales and "in vitro regenerated bulblet" explants on MS medium supplemented with various concentrations of BAP-NAA. The best regeneration on twin scales and "in vitro regenerated bulblets" was obtained on MS medium containing 2 mg l(-1) BAP-0.5 mg 1(-1) NAA and 2 mg 1(-1)BAP-1 mg 1(-1) NAA, respectively. However, bulb scales seemed to be more potent for bulblet regeneration. A large number of the developing bulblets rooted on the regeneration medium. Remaining non-rooting bulblets were rooted on MS medium containing I mg F 1 NAA. All plants were acclimatized in the environmental chamber for 4 weeks and were transferred to the greenhouse for flowering. Regenerated bulblets developed into morphologically normal plants. (c) 2007 Elsevier B.V. All rights reserved.