Amperometric glucose sensor based on the glucose oxidase enzyme immobilized on graphite rod electrode modified with Fe3O4-CS-Au magnetic nanoparticles

Onay A., Doğan Ü., Çiftçi H., Çetin D., Suludere Z., Tamer U.

IONICS, cilt.24, sa.12, ss.4015-4022, 2018 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 24 Sayı: 12
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1007/s11581-018-2559-6
  • Dergi Adı: IONICS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.4015-4022
  • Anahtar Kelimeler: Glucose, Electrochemical sensor, Magnetic nanoparticle, Graphite rod, Chitosan, REDUCED GRAPHENE OXIDE, GLASSY-CARBON ELECTRODE, GOLD NANOPARTICLES, SENSITIVE DETERMINATION, DIRECT ELECTROCHEMISTRY, CHITOSAN, SILVER, ACID, NANOCOMPOSITE, SPECTROSCOPY
  • Gazi Üniversitesi Adresli: Evet

Özet

In this report, an amperometric detection method for blood glucose level was developed benefiting from glucose oxidase (GO) enzyme immobilization on nanoparticle modified graphite rod (GR) electrode. Fe3O4-CS-Au magnetic nanoparticles were synthesized, characterized by TEM, UV-Vis, magnetometry, FTIR, and zeta potential measurements and used for the modification of GR electrode. This modified electrode was used for the detection of glucose level amperometrically at 0.6 V. The obtained calibration graph was linear in the range of 5-30mM glucose concentration with a coefficient of determination (R-2) 0.9971. The limit of detection (LOD) and limit of quantification (LOQ) values were calculated as 0.55 and 1.83mM, respectively. The modified GR electrode showed excellent selectivity in the presence of dopamine, ascorbic acid, and uric acid. The applicability of the developed method was examined in real blood samples by comparing the results obtained from commercial glucose sensor. This novel glucose detection method exhibited fast amperometric response, long storage time, and good selectivity.