In this study, total number of 220 milk and cheese samples were analysed, consisting 120 pasteurised milk samples from 8 different firms, 30 raw milk samples and 70 Turkish white brined cheese samples from 7 different firms which were collected from Ankara region, Turkey. Aflatoxin M1 (AFM1) was determined with an Enzyme Linked Immunosorbent Assay (Elisa) using the I'screen Afla M1 ELISA kit. Sample preparations were done according to the instructions of the Tecna kit. Mean levels of AFM1 was 0.025±0.003 ppb in the milk samples. Regarding the Turkish white brined cheese samples, mean level of AFM1 was found to be 0.023±0.004 ppb. The data revealed that while mean AFM1 levels found in pasteurised milk of two firms were higher than Turkish Food Codex (TFC) values for the other milk was within TFC values. In raw milk and cheese samples all the AFM1 levels were within the TFC. © Medwell Journals, 2010.