Aim: Bacterial aerosols that make up the oral flora, predominantly Staphylococcus aureus (S. aureus), are generated by dental instruments and can remain suspended in the air for significant periods. The present study aimed to determine the distribution of S. aureus aerosols in the immediate environment of dental operators during routine dental treatment and to investigate the reduction in S. aureus colonization using 0.5% hydrogen peroxide/colloidal silver (H2O2/Colloidal-Ag) disinfectant.

Materials and Method: The study, performed on the head phantom’s jaw, was divided into two groups. Sterile distilled water was used in Group A (n = 90 Petri plates). Sterile distilled water with hydrogen peroxide/colloidal silver was used in Group B (n = 90 Petri plates). The plates were set up seperately in two directions on the head phantom corresponding to the 6 and 9 o’clock positions. Three petri plates were placed side by side up to 50 cm, 100 cm and 200 cm distances, and the experimental design were proceeded by contaminating with S. aureus (ATCC 29213). The high-speed dental insturment, which caused aerosol was used for being contamination. We evaluated data from ten-experimental settings for each order. Frequencies, percentages, min, max, mean, median, standart deviation were used to described the data at hand. Mann Whitney U test was used for comparing to independent samples.

Results: Compared to Group A, bacterial colony-forming unit (CFU) numbers of the S. aureus grown colonies on agar plates in Group B was statistically signficantly decreased in directions corresponding to the 6 and 9 o’clock positions to 50 cm, 100 cm, and 200 cm distances (p = 0.00).

Conclusion: H2O2/Colloidal-Ag (0.5%) can safely be used as a supportive precaution against S. aureus within the 6 and 9 o’clock position at dental office.