In this study, the academic staff's job satisfaction and life satisfaction levels of differentiation were formed by their own working conditions between the different variables to determine whether or not the academic staff's working conditions have affected their job satisfaction levels, and in what direction has the effect of life satisfaction been revealed. To measure the relationship between job and life satisfaction, statistical analysis of data collected from 281 other programs were carried out using SPSS 11.0. The reliability of the data analyzed for internal consistency was measured, then the demographic variables associated with the dependent variable for analysis of variance was performed. Finally, correlation and regression analyses were carried out by applying the variable relationship between the "job satisfaction scale" determined by Cronbach's alpha values (0.74) and the "life satisfaction scale" determined by Cronbach's alpha values (0.77). The research was conducted with the survey method. The study sample comprised academic staff in public universities in Ankara Province. The "life satisfaction scale" developed by Diener and Industry (1985) and "job satisfaction scale" developed by Rothen and Brayfield (1951) were used. The results indicated that there is a relationship between job and life satisfaction. Academic staff in terms of job satisfaction shows no difference with regards to gender and task areas, but differences were found between career and marital status. Thus, single staff and professors had higher levels of job satisfaction. Also, the same variables examined in terms of life satisfaction, sex, marital status, career status and responsibilities among the differences in women (unmarried and career high ones) and the health of employees in the life satisfaction levels are higher than that which has been seen.