Effects of iloprost on bleomycin-induced pulmonary fibrosis in rats compared with methyl-prednisolone
Revista Portuguesa de Pneumologia, cilt.18, sa.6, ss.272-277, 2012 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 18 Sayı: 6
- Basım Tarihi: 2012
- Doi Numarası: 10.1016/j.rppneu.2012.04.010
- Dergi Adı: Revista Portuguesa de Pneumologia
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.272-277
- Anahtar Kelimeler: Bleomycin, Iloprost, Methyl-prednisolone, Pulmonary fibrosis, Rats
- Gazi Üniversitesi Adresli: Hayır
Özet
Objective: Prostacyclin (PGI2) has been shown to inhibit the expression of pro-inflammatory and pro-fibrotic mediators in pulmonary fibrosis. In this study, we aimed to test the preventive effects of intraperitoneally administered iloprost, a stable PGI2 analog, on bleomycin-induced pulmonary fibrosis in rats and to compare the effects of iloprost with the effects of methyl-prednisolone, a traditional therapy. Methods: Rats were randomly allocated into four groups: 1. Saline alone (. n=. 6); 2. Bleomycin. +. placebo (. n=. 7); 3. Bleomycin. +. methyl-prednisolone (. n=. 7); 4. Bleomycin. +. iloprost (. n=. 7). Fibrotic changes in the lungs were demonstrated by analyzing the cellular composition of bronchoalveolar lavage fluid, histological evaluation and lung hydroxyproline content. Results: Fibrosis was made in the lungs of rats by bleomycin experimentally. Fibrosis scores in the methyl-prednisolone and the iloprost groups were significantly lower than in the placebo group (. p<. 0.05). Furthermore, the score of the iloprost group was significantly lower than the score of the methyl-prednisolone group. The hydroxyproline content was significantly less in the methyl-prednisolone and the iloprost groups (. p<. 0.05). In the placebo group, the neutrophil percentage in bronchoalveolar lavage was significantly higher than in the other groups, whereas the macrophage percentage in placebo group was significantly lower (. p<. 0.05). Conclusion: Iloprost has protective effect on the pulmonary fibrosis induced by bleomycin and it may be more effective in decreasing fibrotic changes than methyl-prednisolone. © 2011 Sociedade Portuguesa de Pneumologia.