This study was carried out on the chromosomal features of two populations (Kizilirmak and Sakarya Rivers) belonging to Luciobarbus escherichii (Steindachner, 1897). Metaphase chromosomes were obtained from kidney cells. The diploid chromosome number was 2n=100, consisting of 7 pairs of metacentric, 22 pairs of submetacentric and 21 pairs of subtelo-acrocentric chromosomes. The arm number (NF) was 158. Constitutive heterochromatin regions with C-banding were determined on the centromeres of chromosomes. NOR was observed on the short arms of 2 pairs of submetacentric chromosomes. This study is a contribution to cytogenetics of Anatolian cyprinids.