Potential Antioxidant and Enzyme Inhibitory Effects of Nanoliposomal Formulation Prepared from Salvia aramiensis Rech. f. Extract


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ŞEKER KARATOPRAK G., YÜCEL Ç., GÖGER F., Sobarzo-Sanchez E., AKKOL E.

ANTIOXIDANTS, vol.9, no.4, 2020 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 9 Issue: 4
  • Publication Date: 2020
  • Doi Number: 10.3390/antiox9040293
  • Title of Journal : ANTIOXIDANTS

Abstract

Salvia aramiensis Rech. f. is a species that grows only in Hatay, Turkey and is used as a traditional stomachic tea. Neither the chemical composition nor the potential bioactivity of the plant has been investigated before. Antioxidant activity (1,1-Diphenyl-2-picrylhydrazyl Radical (DPPHBLACK CIRCLE) and 2,2'-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS(+BLACK CIRCLE)) radical scavenging and beta-carotene/linoleic acid co-oxidation) of 70% methanol, 70% ethanol extracts, and 2% infusion obtained from S. aramiensis aerial parts were determined. The effect of 70% methanol extract on collagenase and elastase enzyme inhibition and its chemical composition via chromatographic methods (LC-MS/MS and HPLC) were analyzed. Nanoliposomes were developed with 70% methanol extract, were characterized, and were evaluated. The key parameters for the most active 70% methanol extract included the following (DPPHEC50)-E-center dot: 28.4 mu g/mL, Trolox equivalent antioxidant capacity (TEAC)/ABTS: 1.77 +/- 0.09 mmol/L/Trolox. Furthermore 70% methanol extract showed more than 50% inhibition on collagenase and elastase enzymes at all the concentrations. The main component of the extract, rich in phenolic compounds, has been identified as rosmarinic acid; 83.7 mu g/mL extract was released from the nanoliposomal formulation. The extract and its formulation are found to be nontoxic on the L929 fibroblast cell line. This study successfully developed a long-term antioxidant and enzyme inhibitory formulation containing S. aramiensis, which has been used safely among the public for years.