New Nanospheres to Use in the Determination of Imidan Phosmet and Vantex Pesticides


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Karmaz E., Hasanoğlu Özkan E., Yetim N. K., Barutçuoğlu Sarı N.

JOURNAL OF INORGANIC AND ORGANOMETALLIC POLYMERS AND MATERIALS, vol.31, no.7, pp.2915-2924, 2021 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 31 Issue: 7
  • Publication Date: 2021
  • Doi Number: 10.1007/s10904-020-01871-5
  • Journal Name: JOURNAL OF INORGANIC AND ORGANOMETALLIC POLYMERS AND MATERIALS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Chemical Abstracts Core, Chimica, Compendex, INSPEC
  • Page Numbers: pp.2915-2924
  • Keywords: Acetylcholinesterase, Coordination polymer, Pesticide, Nanospheres, Schiff bases
  • Gazi University Affiliated: Yes

Abstract

In this study, the usability of AChE immobilized nanospheres (NSs) was investigated to identify Imidan Phosmet and Vantex pesticides. Accordingly, (2AEPS-SalBr) coded nanosphere was synthesized in result of condensation of (Tris (2 aminoethyl)amine polymer and 5-bromine-2-hydroxy benzaldehyde. Then, new nanosphere coded 2AEPS-SalBr-Pd was synthesized using the template method. The structures of the synthesized nanospheres were characterized by the FT-IR, GPC, SEM/EDX and elemental analysis. Acetylcholinesterase (AChE) enzyme was immobilized to these nanospheres for the determination of Imidan and Vantex. The optimum conditions of the immobilized-AChE enzyme, the number of reuse, storage stability, and the kinetic parameters were determined. The immobilized AChE optimum pH was 8 for both supports, and the optimum temperature was 50 degrees C and 70 degrees C, respectively. Km and Vmax values were calculated as 1.443 mM and 0.251 mM/min for (2AEPS-SalBr)-AChE (pH 8, at 50 degrees C), and 0.680 mM and 0.157 mM/min for (2AEPS-SalBr-Pd)-AChE (pH 8, at 70 degrees C). Then the analyses of Imidan Phosmet and Vantex pesticides were carried out with immobilized AChE on nanospheres. As a result of the research, it was seen that Imidan Phosmet pesticide can be determined at a lower concentration than Vantex pesticide.