Akademik Veri Yönetim Sistemi
13th International Symposium on Pharmaceutical Sciences (ISOPS), Ankara, Türkiye, 22 - 25 Haziran 2021, ss.213-214
Introduction: Bisphenol A (BPA) is a chemical
compound that can bind to estrogen receptors with
low affinity. BPA shows weak estrogenic activity
and is classified as an “endocrine disrupting
chemical” (Almeida et al., 2018). It may also disrupt
thyroid homeostasis (Moriyama et al., 2002).
Selenium has an essential role in thyroid
homeostasis as a component of deiodinases.
Additionally, it protects the thyroid gland against
oxidative stress as a constituent of glutathione
peroxidases (Schomburg 2011). In this study, we
aimed to investigate the toxic effects of BPA and
the modifying effects of selenium supplementation
against BPA toxicity in human papillary thyroid
cancer cell line (B-CPAP). We also evaluated the
cytotoxicity, oxidant/antioxidant parameters,
oxidative DNA damage and apoptosis caused by
BPA. In addition, the protective roles of inorganic
selenium (sodium selenite, SS) and organic
selenium (selenomethionine, SM) were assessed.
Materials and Methods: B-CPAP cell line was
used throughout the experiments. The study
groups were: Control, BPA, SM, SS, BPA+SM and
BPA+SS. Cell viability was assessed by MTT
assay. The oxidative stress parameters including
malondialdehyde (MDA), glutathione (GSH) levels,
intracellular reactive oxygen species (ROS)
production and levels of apoptosis markers
(caspase 3 and 8 l) were measured by using
commercial kits. Oxidative DNA damage was
determined by single cell gel electrophoresis
(COMET) assay. Apoptotic cell count was
evaluated using TUNEL assay.
Results: Our results showed that BPA caused
significant elevations of intracellular ROS and MDA
levels and decreases in total GSH concentrations
in B-CPAP cell line. Additionally, BPA also induced
oxidative DNA damage and apoptosis.
Conclusions: We can conclude that one of the
underlying mechanisms of the toxic effects of BPA
in B-CPAP cell line may be significant alterations of
oxidant/antioxidant status which can lead to DNA
damage. Physiological doses of selenocompounds
might be partially protective against BPA toxicity in
thyroid cell cancer cell lines.
Acknowledgements
13th International Symposıum on Pharmaceutical Sciences, 22-25 June 2021, Ankara/Turkey
214
This study was supported by a grant of Hacettepe
Scientific Research Projects Coordination Unit,
Project number: TYL-2018-17076