Research Journal of Microbiology, cilt.2, sa.1, ss.42-49, 2007 (Hakemli Dergi)
In this study, inactivation’s of some fungal and bacterial
species by Ultraviolet (UV) light were studied under different time and
different surface conditions. We also aimed to determine surface-time relation
in the inactivation of tested microorganisms. The fungi/-bactericidal
and-static effect of UV light at different wavelengths was tested at different
time periods of 1-75 min as well as on different surfaces such as microscope
slide, aluminum foil, coin and solid medium. The distance between the UV light
and the sample was 30 cm. It is the distance widely used in dental and cosmetic
fields in commercial available boxes for sterilization of the tools (e.g.,
scissors, forceps, spatula etc.). It is found that exposure to UV light (254
nm) for 45 min was enough for inactivation of yeast like fungi and bacteria.
Under the same conditions, fungicidal effects against molds were observed in 75
min in all surfaces except for the medium surface. In conclusion, different
surface conditions and exposure time are important in UV light sterilization of
microorganisms.