Relationship between the resistance to bile salts and low pH with exopolysaccharide (EPS) production of Bifidobacterium spp. isolated from infants feces and breast milk


Alp G., ASLIM B.

ANAEROBE, vol.16, no.2, pp.101-105, 2010 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 16 Issue: 2
  • Publication Date: 2010
  • Doi Number: 10.1016/j.anaerobe.2009.06.006
  • Journal Name: ANAEROBE
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.101-105
  • Keywords: Bifidobacterium spp., Probiotic, EPS production, Bile salts, Low pH, LACTIC-ACID BACTERIA, HUMAN GASTROINTESTINAL-TRACT, PROBIOTIC BACTERIA, LACTOCOCCUS-LACTIS, LACTOBACILLUS, TOLERANCE, IDENTIFICATION, POLYSACCHARIDE, SELECTION, SURVIVAL
  • Gazi University Affiliated: Yes

Abstract

The purpose of this study was to investigate a possible relation between resistance to bile salts and low pH with exopolysaccharide (EPS) producing of Bifidobacterium spp. In this study, a total of 31 Bifidobacterium spp. were isolated from breast fed infants feces and breast milk samples. As a result of the identification tests, isolates were identified as Bifidobacterium breve (15 strains), B. bifidum (11 strains), B. pseudocatenulatum (3 strains) and B. longum (2 strains). Bifidobacterium spp. were determined exopolysaccharide (EPS) production. EPS productions observed at chance rations (38.00-97.64 mg/l) among of Bifidobacterium spp. Furthermore, Bifidobacterium spp. were determined resistance to bile salts and low pH. Positive correlations between production of exopolysaccharide and resistance to bile salts (p < 0.01) or low pH (p < 0.01) were found Bifidobacterium spp. This investigation showed that high EPS production of Bifidobacteria may be important in the selection of probiotic strains for resistance to bile salts and low pH. (C) 2009 Elsevier Ltd. All rights reserved.