Genotoxic and Oxidative Damage of Environmental Pollutant Microplastics on Zebrafish (<i>Danio rerio</i>)

Önder S., Koçak G., Günal A. Ç., Sepici Dinçel A.

JOURNAL OF APPLIED TOXICOLOGY, cilt.45, sa.11, ss.2297-2304, 2025 (SCI-Expanded, Scopus) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 45 Sayı: 11
  • Basım Tarihi: 2025
  • Doi Numarası: 10.1002/jat.4846
  • Dergi Adı: JOURNAL OF APPLIED TOXICOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aerospace Database, Applied Science & Technology Source, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chemical Abstracts Core, Chimica, Communication Abstracts, Environment Index, Metadex, Pollution Abstracts, Veterinary Science Database, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.2297-2304
  • Anahtar Kelimeler: DNA damage, microplastic, oxidative damage, zebrafish
  • Gazi Üniversitesi Adresli: Evet

Özet

Microplastics are any synthetic solid particles or polymeric matrices ranging in size from 1 to 5 mm and insoluble in water. This study aimed to evaluate the effects of polystyrene (PS) and polyethylene (PE) microplastics on DNA damage (8-OHdG), advanced oxidation protein products (AOPP mu M/mg protein), malondialdehyde (MDA nmol/g tissue), and glutathione (GSH mu mol/g tissue) in zebrafish. Zebrafish with an average length of 3-5 cm were placed in aquariums filled with spring water with 1-5-mu m PE and 9.5-11.5-mu m PS at 1- and 10-mg/L concentrations for 96 h and 21 days. The tissue levels of MDA exposed to 1-mg/L PS and PE (p = 0.008, p = 0.041) and GSH exposed to 1- and 10-mg/L PE (p = 0.004, p = 0.004) in 96 h were increased compared to the control group. GSH (1 and 10-mg/L PS in 21 days, p = 0.033, p = 0.045) and AOPP levels (1- and 10-mg/L PS in 96 h, p = 0.008, p < 0001) were decreased compared to the control group. DNA damage at 8-OHdG levels (96 h, 10-mg/L PS, p = 0.030) was decreased compared to the control and increased in the 1-mg/L PE at 21 days (p = 0.028). The 1- and 10-mg/L PE and PS microplastic doses were thought to affect the oxidant-antioxidant system. Besides, they may damage DNA in zebrafish, depending on the dosage and exposure time of the chemicals.