Polyvinyl alcohol (PVA) electrospun nanofibers and boric acid (BA) complexes of these nanofibers were synthesized with and without using TritonX-100(TX-100) as a water soluble surfactant (PVA, PVA/BA, PVA/TX-100, PVA/TX-100/BA). The electrospun nanofibers were characterized by SEM, FTIR and TGA. Preliminary results showed that certain amount of boric acid decreased the number of Stapylococcus aureus from 6,8x10(7)(CFU/mL) to 3x10(7)(CFU/mL). In the preparation of polymer, boric acid was used as a crosslinker and antimicrobial agent whereas TX-100 was used as surfactant. The viscosity and conductivity measurements indicated that the BA increased viscosity but decreased conductivity. In contrast, TX-100 increased conductivity without considerable effect on viscosity, which affects the production and resulting nanofiber properties such as diameter and bead formation. The antimicrobial activities of the nanofibers were assayed in vitro by Gram-negative (E. coli) and Gram-positive(S. aureus) bacteria. It was observed that PVA/TX-100/BA showed better antibacterial activity against S.aureus bacteria compared to PVA and PVA/TX-100 nanofibers.