Insights into cholinesterase inhibitory and antioxidant activities of five Juniperus species

Orhan N., ERDOĞAN ORHAN İ., Ergun F.

FOOD AND CHEMICAL TOXICOLOGY, cilt.49, sa.9, ss.2305-2312, 2011 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 49 Sayı: 9
  • Basım Tarihi: 2011
  • Doi Numarası: 10.1016/j.fct.2011.06.031
  • Dergi Adı: FOOD AND CHEMICAL TOXICOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.2305-2312
  • Anahtar Kelimeler: Juniperus, Cupressaceae, Cholinesterase inhibition, Antioxidant activity, Alzheimer's disease, ESSENTIAL OILS, DERIVATIVES, EXTRACTS
  • Gazi Üniversitesi Adresli: Evet

Özet

In vitro acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitory and antioxidant activities of the aqueous and ethanol extracts of the leaves, ripe fruits, and unripe fruits of Juniperus communis ssp. nana, Juniperus oxycedrus ssp. oxycedrus,Juniperus sabina, juniperus foetidissima, and Juniperus excelsa were investigated in the present study. Cholinesterase inhibition of the extracts was screened using ELISA microplate reader. Antioxidant activity of the extracts was tested by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and superoxide radical scavenging, ferrous ion-chelating, and ferric-reducing antioxidant power (FRAP) assays. Total phenol and flavonoid contents of the extracts were determined spectrophotometrically. The extracts had low or no inhibition towards AChE, whereas the leaf aqueous extract of J. foetidissima showed the highest BChE inhibition (93.94 +/- 0.01%). The leaf extracts usually exerted higher antioxidant activity. We herein describe the first study on anticholinesterase and antioxidant activity by the methods of ferrous ion-chelating, superoxide radical scavenging, and ferric-reducing antioxidant power (FRAP) assays of the mentioned Juniperus species. (C) 2011 Elsevier Ltd. All rights reserved.