A Novel Amperometric Biosensor for Detection of Bisphenol A

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Bodur O. C., Keskin M., Arslan Avan B., Arslan H.

3. EBAT/ Eurasia Biochemical Approaches & Technologies Congress, Antalya, Türkiye, 4 - 07 Kasım 2021, ss.154

  • Yayın Türü: Bildiri / Özet Bildiri
  • Basıldığı Şehir: Antalya
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.154
  • Gazi Üniversitesi Adresli: Evet

Özet

Bisphenol A, 2,2-Bis-4-hydroxyphenyl-propane, (BPA) is a monomer commonly used in the production of epoxy resins and polycarbonate plastics. BPA plastics are frequently used in cups, home food containers and dental filling materials. Due to its chemical structure, BPA shows activity similar to the endocrine hormones estradiol and diethylstilbestrol1. BPA and its derivatives can bind to estrogen receptors and cause neurological disturbances, even at low doses, where it acts similar to estrogen. Therefore, it is important to determine BPA and its derivatives quickly and sensitively at low concentrations1.

In this study, the amount of BPA in various materials used in daily life was determined with an amperometric tyrosinase biosensor. Commercial Fe3O4 nanoparticles were used to modify carbon paste electrode (MCPE).  Tyrosinase was immobilized on MCPE by cross-linking method with glutaraldehyde. BPA is oxidized to p-bisbenzoquinone by an enzymatic reaction catalyzed by tyrosinase in the presence of oxygen. BPA was quantified by measuring the electrochemical reduction of the p-bisbenzoquinone compound formed as a result of the enzymatic reaction at -0.15 V. Then, the optimal working conditions necessary for the BPA biosensor were investigated and the linear operating range of the biosensor was determined. In order to determine the optimum operating conditions of the biosensor, the efect of pH and temperature was investigated. Optimum pH and temperature were found to be 5.0 and 40 oC, respectively. The storage stability and application stability of the biosensor were also studied. Several possible interfering substances’ effects on the BPA biosensor were investigated. The developed biosensor was tested in determinations of BPA amount in a real sample.