Determination of endocrine disrupting effects of the antifouling pyrithiones on zebrafish (Danio rerio)


Günal A. Ç. , Arslan P., İpiçürük N., Tural R., Sepici Dinçel A.

Energy, Ecology and Environment, vol.7, no.5, pp.523-531, 2022 (Peer-Reviewed Journal) identifier identifier

  • Publication Type: Article / Article
  • Volume: 7 Issue: 5
  • Publication Date: 2022
  • Doi Number: 10.1007/s40974-022-00245-6
  • Journal Name: Energy, Ecology and Environment
  • Page Numbers: pp.523-531
  • Keywords: Danio rerio, Zinc pyrithione, Copper pyrithione, Endocrine disruptors, Histological alterations, Vitellogenin, FRESH-WATER FISH, ZINC PYRITHIONE, COPPER PYRITHIONE, ACUTE TOXICITY, OREOCHROMIS-NILOTICUS, KIDNEY HISTOPATHOLOGY, GILL, VITELLOGENIN, MEDAKA, PESTICIDES
  • Gazi University Affiliated: Yes

Abstract

© 2022, The Joint Center on Global Change and Earth System Science of the University of Maryland and Beijing Normal University.Bacteria, algae, and fungi are the organisms affected by biocidal substances, which are widely used. Apart from these, they can also have toxic effects on various aquatic organisms. Copper pyrithione (CPT) and zinc pyrithione (ZPT) are used as an alternative to tributyltin, which is forbidden and known to be highly toxic to the aquatic ecosystem. However, there is lacking information about histopathological alterations and endocrine disrupting effects of these substances. Therefore, this study was aimed to investigate the effects of ZPT, CPT, and their mixtures on the histological changes in the tissues and on the vitellogenin hormone in male zebrafish. Substances caused hyperemia, epithelial lifting, telangiectasis, and hyperplasia in the gill tissue. In the liver tissue, hyperemia, hydropic degeneration and necrosis were observed. In addition, apoptosis was found in the gill, liver, and testicle of the tissues as a result of the TUNEL assay. Vitellogenin hormone, on the other hand, increased in the experimental groups compared to the control groups (p < 0.05). According to the obtained results, these substances, which are used as alternative antifouling materials, show as endocrine disrupting compounds by acting on vitellogenin as well as causing histopathological changes.