Climate change necessitates reducing fossil fuels and increasing renewable energy sources. On the other hand, the COVID-19 process has increased the need for disinfectants and the use of alcohol as fuel is prohibited. fusel oil, which cannot be used as a disinfectant, is a very smelly waste alcohol mixture left after the production of ethyl alcohol from beet molasses in sugar factories. In this study, fusel oil and synthetic manganese additives in different volumetric ratios were added to gasoline and their effects on engine performance, specific fuel consumption and emissions were investigated.5%, 10%, 15% and 20% were determined as volumetric fuel mixtures.In tests based on the viscosity, freezing points and flash points of the mixtures, adding 15% fusel oil to gasoline under laboratory conditions was determined as the most suitable ideal mixture (F15B85). 4ppmF15B85, 8ppmF15B85, 12ppmF15B85, 16ppmF15B85 measurement fuels were formed by adding 4-8-12 and 16 ppm organic resin-based manganese additives into Fl5B85 test fuel, respectively. The engine performance and emission values were examined by making measurement tests in an atmospheric charged gasoline engine with the measurement fuels obtained. According to the experimental results, 12ppmF15B85 fuel gave positive results in terms of engine performance and emissions compared to other fuel mixtures. An average of 4.18% increase in torque, 5.04% increase in power, 3.12% increase in BSFC (Brake Specific Fuel Consumption) and 2.48% decrease in EGT (Exhaust Gas Temperature) were determined in engine performances. In terms of exhaust emissions, an average of 20.69%, 10.51% reductions in CO (carbon monoxide) and HC (hydrocarbon), respectively, and an increase of 3.65% and 5.10% in CO2 (Carbon dioxide) and NOx (nitrous oxide) were detected.