Effects Of The Sevoflurane And Magnesium On Maternal Oxidative Stress, İnflammation And Fetal Brain Histopathology

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Özdemir Ç., Koca G., İnan M. A., Işık B.

ASPA 2022, İstanbul, Turkey, 14 - 16 October 2022, pp.140-142

  • Publication Type: Conference Paper / Full Text
  • City: İstanbul
  • Country: Turkey
  • Page Numbers: pp.140-142
  • Gazi University Affiliated: Yes


INTRODUCTION/ PURPOSE: Studies have demonstrated that exposure to anesthetic agents during neurodevelopment triggers neurodegeneration and subsequent long-term neurobehavioral abnormalities(1). The developing brain is vulnerable to the harmful effects of anesthetics. Magnesium sulfate has been shown to protect the nervous system from inflammation and ischemic episodes(2-3). We aimed to evaluate the effects of sevoflurane and/or MgSO4, the most commonly used anesthetic and sedative agent in obstetric anesthesia, on maternal oxidative stress and fetal brain histopathology in midgestational rats. MATERIALS and METHOD: Pregnant rats were randomly divided into 4 groups on the 14th. day. Group C (control) were exposed to 100% oxygen for 2 hours. Group Sv (sevoflurane) were also exposed to 2.3% sevoflurane with oxygen for 2 hours. MgSO4 was injected intraperitoneally at a dose of 270 mg/kg to the rats in Group Sv+MgSO4 and group MgSO4. Sevoflurane exposure in Group Sv+MgSO4 started 30 minutes after injection. Inflammatory and oxidative markers (as an IL-6, IL-10, TNF-α, TAS, TOS, OSİ) and histopathological evaluation were performed in maternal blood or fetal brain tissue. FINDINGS: Rats in Group Sv had higher serum TNF-α [159.641pg/mL (Median)] compared to Group C [71,943 pg/ml (Median)] (P=0.04). There was no significant difference in IL-6, IL-10 and serum TAS, TOS and OSI (P<0.05) Results are shown in Table 1. In the histopathological evaluation of fetal brains, no findings suggesting damage pathways such as apoptosis, autophagy, and inflammation were found. DISCUSSION / CONCLUSION: In pregnant rats, exposure to anesthetic doses of sevoflurane during mid-pregnancy did not cause an increase in inflammatory and oxidative stress markers other than TNF-α in maternal rats and histopathological changes in fetal brain tissue. For this reason, in clinical dose range neither sevoflurane nor MgSO4+sevoflurane were not negative effect on maternal oxidative stress or fetal histopathological changes. But, TNF-alpha elevation in Group Sv should be taken into account.